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Влияние гипербарической оксигенации на глутаминовый цикл в оперированной печени
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Savilov P.N. The effect of hyperbaric oxygenation on glutamine cycle in the operated liver. Gastroenterology. Surgery. Intensive care. Consilium Medicum. 2019; 3: 16–20. DOI: 10.26442/26583739.2019.3.190488
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Ключевые слова: гипероксия, глутамин, аммиак, резекция печени, глутаминовый цикл.
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The effect of hyperbaric oxygenation (HBO) on the glutamine cycle in hepatocytes of the operated liver was studied in 136 Mature white rats weighing 180–220 g. Operation of the liver resection (LR), which was carried out under ether anesthesia, removing the left part of the organ with an electric knife. HBO (3 ATA, 50 min) was performed 8–14, 24 and 48 hours after LR. The object of the study was the remaining after resection part of the left lobe of liver (LLL), non-operated middle lobe of the liver (MLL), arterial blood, portal vein and hepatic veins, where the content of ammonia (Am), glutamine (Gn), glutamine synthetase activity (HS), phosphate-dependent glutaminase (PDG) was studied. The studies were performed on the 3rd day of the postoperative period. The studies were performed on the 3rd day of the postoperative period. It was found that in a three-day course of HBO eliminates the violation of the formation of "hepatic" Gn in the LLL and activates this reaction in the MLL by stimulating the GS of her hepatocytes. This is accompanied by the normalization of the liver's Gn-endocrine function. Encouraging the development of portal hyperglutaminemia, HBO creates the conditions for the activation of the cookies PDG catalyzing the dezamidation of the Gn coming to her with arterial and portal blood. This prevents the accumulation of Am in the operated organ and its increased increment from it into the blood, which leads to the elimination of arterial hyperammonemia.
Key words: hyperoxia, glutamine, ammonia, liver resection, glutamine cycle.
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1. Häussinger D. Zonirung der hepatischen Ammoniak- und Glutaminschtoffwechsels und Bedeutung der intracellularen Glutaminzyclus bei der Ammoniumionenentgiftung. Der Krankenhausarzt 1984; 57 (3): 202–8.
2. Häussinger D, Sies H, Gerok W. Leber und pH-Regulation: Die Bedeutung der intracellularen Glutaminzyklus In.: Verchandlungen der Deutschen Gesellschaft fur innere. Medizine 1984; 90: 1165–7.
3. Savilov P.N. Ammonia neutralization function of hepaticytes after liver resection in experiment. Patol. fiziologiia i eksperim. terapiia. 2002; 4: 11–3 (in Russia).
4. Savilov P.N. Glutamine cycle of the liver after resection.. Vopr. biologicheskoi, meditsinskoi i farmatsevticheskoi khimii. 2010; 7: 53–8 (in Russia).
5. Savilov P.N. Ammoniumdetoxications function of liver in chronic active hepatitis. Patol. fiziologiia i eksperim. terapiia. 2004; 1: 24–6 (in Russia).
6. Savilov P.N. Influence of partial hepatectomie on ammoniumdetoxications function of liver at chronic tetrachlocarbon hepatitis. Patol. fiziologiia i eksperim. terapiia. 2017; 61 (2): 61–6 (in Russia).
7. Malutin V.E. Glutamin metabolism in liver in early postterminal period under hyperbaric oxygenation. Biul. giperbaricheskoi biologii i meditsiny. 1993; 1 (1–4): 26–30 (in Russia).
8. Savilov P.N., Moltchanov D.V. Impact of Hyperbaric Oxyganation on Renal Ammonia Excretion during Experimental Liver Resection. Obshchaia reanimatologiia. 2015; 11 (2): 56–63 (in Russia).
9. Savilov P.N., Kuz'mina N.I., D'iachkova S.Ia. Materials for the study of antimicrobial liver function of a healthy body. Vestn. VGU. Problemy khimii, biologii. 2002; 1: 41–3 (in Russia).
10. Silakova A.I., Trubin G.P., Iavlikova A.I. Micromethod for ammonia and glutamine identification in trichloroacetic tissue extracts. Vopr. meditsinskoi khimii. 1962; 8 (5): 538–44 (in Russia).
11. Harris M. Studies regenerating a glutamine-like substance in blood and spinal fluid, including a method for its quantitative determination. J Clin Invest 1943; 22 (4): 569–76.
12. Keller H, Muller-Beisenritz M, Neumann E. Eine Methode zur Ammoniakbestimmung in Capillarblut. Klin Wsch 1967; 15: 314–9.
13. Jonson D, Lardy I. Method in Enzimology. NY 1972; 10: 94–102.
14. Beaton JR, Ozava G. Activity of liver glutaminazez in vitamin B6-dificient rats. J Biol Chemistry 1955; 214 (2): 685–91.
15. Pushkin V.A., Evstigneeva Z.G., Kretovich V.L. Determination of the glutaminsintetazes activity in extracts from pea seeds on the formation of orthophosphate. Prikladnaia biokhimiia i mikrobiologiia. 1972; 3 (1): 90–6 (in Russia).
16. Hartree EF. Determination of protein a modification of the Loury method that gives a linear photometric responds. Anal Biochem 1972; 43 (2): 422–7.
17. Glantz StA. Primer of Biostatistics. N.Y.: McGraw-Hill Inc, 1994.
18. Solopaev B. P. Regeneration of normal and pathologically changed liver. Gor'kii: Verkhne-Volzhsk. izd-vo, 1980 (in Russia).
19. Kozlov B.A., Kovalenko H.A. Glutaminazes. Uspekhi biologicheskoi khimii.1972; 13: 40–79 (in Russia).
20. Savilov P.N., Molchanov D.V., Iakovlev V.N. Impact of hyperbaric oxygenation on glutamine kinetics in hepatic failure (an experimental study). Obshchaia reanimatologiia. 2012; 8 (2): 20–7 (in Russia).
ТОГБУЗ «Тамбовская центральная районная больница», с. Покрово-Пригородное, Россия;
ФГБОУ ВО «Воронежский государственный медицинский университет им. Н.Н. Бурденко» Минздрава России, Воронеж, Россия
*p_savilov@mail.ru
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Pavel N. Savilov*
Tambov Central District Hospital, v. Pokrovo-Prigorodnoye, Russia; Burdenko Voronezh State Medical University, Voronezh, Russia
*p_savilov@mail.ru