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Критерии оценки качества ооцита в циклах ИКСИ: взгляд клинического эмбриолога
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Аннотация
В циклах стимуляции суперовуляции часто можно видеть появление ооцитов с различными повреждениями. Аномалии ооцита можно условно разделить на две группы: экстрацитоплазматические и цитоплазматические. К экстрацитоплазматическим аномалиям относят нарушение строения и формы зоны пеллюцида, дебрис в перивителлиновом пространстве и его резкое увеличение, аномалии первого полярного тела (увеличение в размере, дегенерация, мультифрагментация), а также нарушение симметрии оолеммы ооцита. К цитоплазматическим аномалиям, определяемым на световом уровне при оценке ооцита, относят гранулярность цитоплазмы клетки, агрегаты гладкого эндоплазматического ретикулума, рефрактерные тела, вакуоли, нарушение вязкости цитоплазмы.
В статье рассматриваются критерии оценки качества ооцита в циклах ИКСИ, а также прогноз развития патологичных клеток.
Ключевые слова: ИКСИ, морфология ооцита, качество ооцита.
В статье рассматриваются критерии оценки качества ооцита в циклах ИКСИ, а также прогноз развития патологичных клеток.
Ключевые слова: ИКСИ, морфология ооцита, качество ооцита.
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The appearance of oocytes with injuries occurs during the ovular stimulation. Oocyte dysmorphisms can be divided into two groups: extracytoplasmic and cytoplasmic. Extracytoplasmic anomalies include a violation of the structure and shape of the zone pellucida, debris in the perivitelline space and its dramatic increase, abnormal first polar body (increased size, degeneration, multifragmentation) as well as breaking of the ovolemma symmetry. Defined on the light level cytoplasmic anomalies are the oocyte cytoplasm granularity, smooth endoplasmic reticulum clusters, refractile bodies, vacuoles, and increased viscosity of the cytoplasm.
This article discusses the criteria for evaluating the quality of oocytes in ICSI, and forecast of the development of pathological cells.
Key words: ICSI, oocyte morphology, oocyte quality.
Полный текст
Список литературы
1. Balaban B, Ata B, Isiklar A et al. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod 2008; 23: 1778–85.
2. Bell CE, Calder MD, Watson AJ. Genomic RNA profiling and the programme controlling preimplantation mammalian development. Mol Hum Reprod 2008; 14 (12): 691–701.
3. Bluhm BA, Brey T, Klages M et al. Occurrence of the autofluorescent pigment, lipofuscin, in polar crustacean and its potential as an age marker. Polar Biol 2001; 24: 642–9.
4. Caballero-Campo P, Chirinos M, Fan XJ et al. Biological effects of recombinant human zona pellucida proteins on sperm function. Biol Reprod 2006; 74: 760–8.
5. Chiu P, Wong B, Lee CL et al. Native human zona pellucida glycoproteins: purification and binding properties. Hum Reprod 2008; 6 (23): 1385–93.
6. Conner SJ, Lefievre L, Hughes DC et al. Cracking the eggs: increased complexity in the zona pellucida. Hum Reprod 2005; 20: 1148–52.
7. Ebner T, Moser M, Sommergruber M et al. Occuarance and developmental consequences of valuoles throughout preimplantation development. Fertil Steril 2005;83: 1635–40.
8. Ebner T, Moser M, Sommergruber M et al. Developmental competence of oocytes showing increased cytoplasmic viscosity. Hum Reprod 2003; 18 (6): 1294–8.
9. Ebner T, Shebl O, Moser M et al. Developmental fate of ovoid oocytes. Hum Reprod 2008; 23 (1): 62–6.
10. Hassan-Ali H, Hisham-Saleh A, El-Gezeiry D et al. Perivitelline space granularity: a sign of human menopausal gonadotrophin overdose in intracytoplasmic sperm injection. Hum Reprod 1998; 13 (12): 3425–30.
11. Kahraman S, Yakin K, Donmez E et al. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum Reprod 2000; 15: 2390–3.
12. Kaji K, Kudo A. The mechanism of sperm-oocyte fusion in mammals. Reproduction 2004; 127: 423–9.
13. Kiefer SM, Saling P. Proteolytic processing of human zona pellicida proteins. Biol Reprod 2002; 66: 407–14.
14. Lefievre L, Conner SJ, Salpekar A et al. Four zona pellucida glycoproteins are expressed in the human. Hum Reprod 2004; 19: 1580–6.
15. Levi M, Kaplan-Kraicer R, Shalgi R. Regulation of division in mammalian oocytes: implications for polar body formation. Mol Hum Reprod 2011; 17 (5): 328–34.
16. Magerkurth C, Topfer-Petersen E, Schwartz P et al. Scanning electron microscopy analysis of the human zona pellucida: influence of maturity and fertilzation on morphology and sperm binding pattern. Hum Reprod 1999; 14 (4): 1057–66.
17. Mannikko M, Tormala R-M, Tuuri T et al. Association between sequence variations in genes encoding human zona pellucida glycoproteins and fertilization failure in IVF. Hum Reprod 2005; 20 (6): 1578–85.
18. Otsuki J, Okada A, Morimoto K et al. The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod 2004; 19 (7): 1591–7.
19. Otsuki J, Nagai Y, Chiba K. Lipofuscin bodies in human oocytes as an indicator of oocyte quality. J Assist Reprod Genet 2007; 24: 263–70.
20. Van Blerkom J, Davis PW, Lee J. ATP content of human oocytes and developmental potential and outcome after in-vitro fertilization and embryo transfer. Hum Reprod 1995; 10: 415–24.
21. Veeck L. Atlas of the human oocyte and early conceptus. Baltimore: Williams&Wilkins, 1991.
22. Wassarman PM, Jovine L, Litscher ES. A profile of fertilization in mammals. Nat Cell Biol 2001; 3: 59–64.
2. Bell CE, Calder MD, Watson AJ. Genomic RNA profiling and the programme controlling preimplantation mammalian development. Mol Hum Reprod 2008; 14 (12): 691–701.
3. Bluhm BA, Brey T, Klages M et al. Occurrence of the autofluorescent pigment, lipofuscin, in polar crustacean and its potential as an age marker. Polar Biol 2001; 24: 642–9.
4. Caballero-Campo P, Chirinos M, Fan XJ et al. Biological effects of recombinant human zona pellucida proteins on sperm function. Biol Reprod 2006; 74: 760–8.
5. Chiu P, Wong B, Lee CL et al. Native human zona pellucida glycoproteins: purification and binding properties. Hum Reprod 2008; 6 (23): 1385–93.
6. Conner SJ, Lefievre L, Hughes DC et al. Cracking the eggs: increased complexity in the zona pellucida. Hum Reprod 2005; 20: 1148–52.
7. Ebner T, Moser M, Sommergruber M et al. Occuarance and developmental consequences of valuoles throughout preimplantation development. Fertil Steril 2005;83: 1635–40.
8. Ebner T, Moser M, Sommergruber M et al. Developmental competence of oocytes showing increased cytoplasmic viscosity. Hum Reprod 2003; 18 (6): 1294–8.
9. Ebner T, Shebl O, Moser M et al. Developmental fate of ovoid oocytes. Hum Reprod 2008; 23 (1): 62–6.
10. Hassan-Ali H, Hisham-Saleh A, El-Gezeiry D et al. Perivitelline space granularity: a sign of human menopausal gonadotrophin overdose in intracytoplasmic sperm injection. Hum Reprod 1998; 13 (12): 3425–30.
11. Kahraman S, Yakin K, Donmez E et al. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum Reprod 2000; 15: 2390–3.
12. Kaji K, Kudo A. The mechanism of sperm-oocyte fusion in mammals. Reproduction 2004; 127: 423–9.
13. Kiefer SM, Saling P. Proteolytic processing of human zona pellicida proteins. Biol Reprod 2002; 66: 407–14.
14. Lefievre L, Conner SJ, Salpekar A et al. Four zona pellucida glycoproteins are expressed in the human. Hum Reprod 2004; 19: 1580–6.
15. Levi M, Kaplan-Kraicer R, Shalgi R. Regulation of division in mammalian oocytes: implications for polar body formation. Mol Hum Reprod 2011; 17 (5): 328–34.
16. Magerkurth C, Topfer-Petersen E, Schwartz P et al. Scanning electron microscopy analysis of the human zona pellucida: influence of maturity and fertilzation on morphology and sperm binding pattern. Hum Reprod 1999; 14 (4): 1057–66.
17. Mannikko M, Tormala R-M, Tuuri T et al. Association between sequence variations in genes encoding human zona pellucida glycoproteins and fertilization failure in IVF. Hum Reprod 2005; 20 (6): 1578–85.
18. Otsuki J, Okada A, Morimoto K et al. The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod 2004; 19 (7): 1591–7.
19. Otsuki J, Nagai Y, Chiba K. Lipofuscin bodies in human oocytes as an indicator of oocyte quality. J Assist Reprod Genet 2007; 24: 263–70.
20. Van Blerkom J, Davis PW, Lee J. ATP content of human oocytes and developmental potential and outcome after in-vitro fertilization and embryo transfer. Hum Reprod 1995; 10: 415–24.
21. Veeck L. Atlas of the human oocyte and early conceptus. Baltimore: Williams&Wilkins, 1991.
22. Wassarman PM, Jovine L, Litscher ES. A profile of fertilization in mammals. Nat Cell Biol 2001; 3: 59–64.
________________________________________________
1. Balaban B, Ata B, Isiklar A et al. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod 2008; 23: 1778–85.
2. Bell CE, Calder MD, Watson AJ. Genomic RNA profiling and the programme controlling preimplantation mammalian development. Mol Hum Reprod 2008; 14 (12): 691–701.
3. Bluhm BA, Brey T, Klages M et al. Occurrence of the autofluorescent pigment, lipofuscin, in polar crustacean and its potential as an age marker. Polar Biol 2001; 24: 642–9.
4. Caballero-Campo P, Chirinos M, Fan XJ et al. Biological effects of recombinant human zona pellucida proteins on sperm function. Biol Reprod 2006; 74: 760–8.
5. Chiu P, Wong B, Lee CL et al. Native human zona pellucida glycoproteins: purification and binding properties. Hum Reprod 2008; 6 (23): 1385–93.
6. Conner SJ, Lefievre L, Hughes DC et al. Cracking the eggs: increased complexity in the zona pellucida. Hum Reprod 2005; 20: 1148–52.
7. Ebner T, Moser M, Sommergruber M et al. Occuarance and developmental consequences of valuoles throughout preimplantation development. Fertil Steril 2005;83: 1635–40.
8. Ebner T, Moser M, Sommergruber M et al. Developmental competence of oocytes showing increased cytoplasmic viscosity. Hum Reprod 2003; 18 (6): 1294–8.
9. Ebner T, Shebl O, Moser M et al. Developmental fate of ovoid oocytes. Hum Reprod 2008; 23 (1): 62–6.
10. Hassan-Ali H, Hisham-Saleh A, El-Gezeiry D et al. Perivitelline space granularity: a sign of human menopausal gonadotrophin overdose in intracytoplasmic sperm injection. Hum Reprod 1998; 13 (12): 3425–30.
11. Kahraman S, Yakin K, Donmez E et al. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum Reprod 2000; 15: 2390–3.
12. Kaji K, Kudo A. The mechanism of sperm-oocyte fusion in mammals. Reproduction 2004; 127: 423–9.
13. Kiefer SM, Saling P. Proteolytic processing of human zona pellicida proteins. Biol Reprod 2002; 66: 407–14.
14. Lefievre L, Conner SJ, Salpekar A et al. Four zona pellucida glycoproteins are expressed in the human. Hum Reprod 2004; 19: 1580–6.
15. Levi M, Kaplan-Kraicer R, Shalgi R. Regulation of division in mammalian oocytes: implications for polar body formation. Mol Hum Reprod 2011; 17 (5): 328–34.
16. Magerkurth C, Topfer-Petersen E, Schwartz P et al. Scanning electron microscopy analysis of the human zona pellucida: influence of maturity and fertilzation on morphology and sperm binding pattern. Hum Reprod 1999; 14 (4): 1057–66.
17. Mannikko M, Tormala R-M, Tuuri T et al. Association between sequence variations in genes encoding human zona pellucida glycoproteins and fertilization failure in IVF. Hum Reprod 2005; 20 (6): 1578–85.
18. Otsuki J, Okada A, Morimoto K et al. The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod 2004; 19 (7): 1591–7.
19. Otsuki J, Nagai Y, Chiba K. Lipofuscin bodies in human oocytes as an indicator of oocyte quality. J Assist Reprod Genet 2007; 24: 263–70.
20. Van Blerkom J, Davis PW, Lee J. ATP content of human oocytes and developmental potential and outcome after in-vitro fertilization and embryo transfer. Hum Reprod 1995; 10: 415–24.
21. Veeck L. Atlas of the human oocyte and early conceptus. Baltimore: Williams&Wilkins, 1991.
22. Wassarman PM, Jovine L, Litscher ES. A profile of fertilization in mammals. Nat Cell Biol 2001; 3: 59–64.
Авторы
Н.П.Макарова, Е.А.Калинина
ФГБУ Научный центр акушерства, гинекологии и перинатологии им. акад. В.И.Кулакова Минздравсоцразвития РФ
ФГБУ Научный центр акушерства, гинекологии и перинатологии им. акад. В.И.Кулакова Минздравсоцразвития РФ
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